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The most important contribution made through PTC is the demonstration of the unique capacity of plant cells to regenerate full plants, via organogenesis or embryogenesis, irrespective of their source (root, leaf, stem, floral parts, pollen, endosperm) and ploidy level (haploid, diploid, triploid). PTC involves a scientific approach to systematically optimize physical (nature of the substrate, pH, light, temperature and humidity), chemical (composition of the culture medium, particularly nutrients and growth regulators), biological (source, physiological status and size of the explant), and environmental (gaseous environment inside the culture vial) parameters to achieve the desired growth rate, cellular metabolism, and differentiation. It also requires an experienced and vigilant eye to select healthy and normal tissues for subculture. It is the art of growing experimental plants, selecting a suitable plant organ or tissue to initiate cultures, cleaning, sterilization and trimming it to a suitable size, and planting it on a culture medium in right orientation while maintaining complete asepsis. Plant tissue culture (PTC) broadly refers to cultivation of plant cells, tissues, organs, and plantlets on artificial medium under aseptic and controlled environmental conditions. (Edinburgh), the visionary Founder Director of the Dayalbagh Educational Institute, for the inspiration and strength to undertake and complete the task of writing this book Printed on acid-free paper Springer is part of Springer Science+Business Media (Dedicated to the most Revered Dr. The publisher makes no warranty, express or implied, with respect to the material contained herein. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made.
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Sant Saran Bhojwani Prem Kumar Dantu Department of Botany Dayalbagh Educational Institute Agra, Uttar Pradesh India
Plant Tissue Culture: An Introductory Text
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